Are you a mature character or just a child? Take down three enemies of the same kind in order to trigger mega-jump bonuses! Collect banana shields to plow through your opponents in style! But remember to watch out for bamboo signs with different messages, red agile monkeys, parrots and gorillas because they will knock you off and send you to your tropical doom!
He didn't, and the two remained apart. When the movie was released, and Mike's ex viewed the movie with her new steady boyfriend, she was mortified not only to learn that the gun-rack anecdote had been written into the film, but also she was shocked to see that the main characters referred to the Stacy character as a 'psycho hose-beast'.
Some time later, Myers telephoned his former girl, attempting to apologize for including a very detracting version of her in the movie. The film is credited with reviving the popularity of the British rock band Queen in the United States through its use of their song "Bohemian Rhapsody". They had dropped in popularity throughout the s in the US and hadn't even bothered to include the country in their final two international tours with lead singer and the song's writer Freddie Mercury for their album "The Works" and their album "A Kind of Magic".
Wayne's World caused the song to become a bigger hit in the US chart number two than it had been first time around number nine. By a remarkable coincidence, Mercury didn't live to see the song's renaissance as he had become rock's most famous AIDS casualty just a few months before the film's release.
However, according to guitarist Brian May , Mercury did give permission for the song to be used and saw the clips while he was close to death because Mike Myers had sent a tape and wanted him to see it. In the early '70s, some British music shops banned or fined patrons for playing Led Zeppelin 's "Stairway to Heaven" because it was played so often.
Hence the sign: "No 'Stairway to Heaven'" when Wayne plays the guitar in the store. Mike Myers originally didn't want to share the limelight with Dana Carvey. Myers had originally developed the Wayne character as a solo character while performing with Second City. The character of Garth was added for Saturday Night Live At the time, Carvey was arguably the bigger star. Rob Lowe has said he discovered his "hitherto untapped gift for comedy" after meeting Mike Myers , who would later cast him in the Austin Powers sequels.
The film is credited with reviving his career following the sex-tape scandal. Garth describes a wholly made up The Twilight Zone story. Dana Carvey did not learn the lyrics to "Bohemian Rhapsody" prior to filming the scene where everyone is singing along to it, and was reportedly displeased with the take of that scene used in the film because he was obviously not singing, just moving his mouth in vague relation to the lyrics.
Tia Carrere sang all her own vocals on songs she performed in the film, and her cover songs, such as Sweet 's "The Ballroom Blitz", were included on the film's soundtrack album.
While filming the "Bohemian Rhapsody" sequence, both Mike Myers and Dana Carvey developed severe pain in their necks from all the head banging. There are scenes later in the movie where it becomes apparent they are trying to move their necks as little as possible. The scene with Wayne and Garth talking on the hood of their car was the last scene filmed. Since everyone was tired and just wanted the movie done, they ad-libbed it.
The "Stairway to Heaven" guitar riff was changed for the international, cable, and videotape releases to a generic riff because of disputes in obtaining rights to the first five notes of the song, which appear only in the U. Dana Carvey modeled Garth after his brother Brad. Dana Carvey stated in interviews to promote this film during its theatrical run that he developed severe pain in his jaw due to Garth's overbite, and that he spent his nights after filming had wrapped for the day with bags of ice held to the sides of his face.
According to Penelope Spheeris , Mike Myers was difficult to work with. He arrived on set one day to discover that the snack table only had butter and not margarine for his bagel. Myers reportedly became enraged, flipped the table over, stormed off the set, and did not come out of his trailer for hours. She assigned her daughter to be his assistant. She told Entertainment Weekly: "He Myers was emotionally needy and got more difficult as the shoot went along. Why do we have to do this so many times?
No one is going to laugh at that! Donna Dixon Garth's dream woman has been Dan Aykroyd 's wife since Mike Myers said filming was "a blur," because his father's health was dwindling at the same time. The movie was shot in 34 days. Mike Myers said in that he didn't think including the Terminator 2: Judgment Day reference would be funny, but that "people went shithouse over it.
Mike Myers and Penelope Spheeris argued over the final cut of the film. Myers then blocked Spheeris from directing the sequel. A teaser trailer for the film was released in November preceding The Addams Family , where Wayne and Garth sing the Addams Family theme song in the grave-yard set of that movie. Mike Myers 's feature film debut. Wayne Campbell's uniquely American slang proved a challenge for translators into foreign languages Wayne's line "And monkeys might fly out of my butt!
Mike Myers appears in Bohemian Rhapsody as an EMI executive who refuses to air "Bohemian Rhapsody" because it's too long and kids need a song that they can "bang their heads to in a car. Additionally, the inclusion of "Bohemian Rhapsody" in "Wayne's World" was responsible for Queen 's resurgence in the U.
The first two are Ted Nugent live albums and the third is a Cheap Trick live album. When the plane flies over Wayne and Garth, the plane seen from underneath is actually a four-foot-long model. Tia Carrere turned down a part on Baywatch to star in the film.
Two "No Stairway to Heaven" signs were made for the film. The one used in the movie spells out the whole phrase, while a second one, not used in the film, simply read "Stairway to Heaven" and had a "no cross" through it similar to "no parking" signs. The one not used in the movie was featured on Pawn Stars, being sold by someone who had worked in the guitar shop used in the movie, along with some other Wayne's World memorabilia.
Cassandra's horrible jungle themed music video is a possible reference to Pearl Jam , who reportedly went through the same thing for Pearl Jam: Even Flow They were so unhappy with the finished result that they prevented its release and swore off music videos forever.
To determine if antioxidant physiology is dependent on Cd exposure route in the mayfly Centroptilum triangulifer, we exposed larvae to environmentally relevant concentrations of Cd from isolated dissolved or dietary exposure routes to achieve comparable tissue concentrations.
Dissolved Cd had no effect on the antioxidant enzymes examined. However, dietary Cd significantly suppressed catalase and superoxide dismutase activities, and decreased concentrations of the reduced active form of glutathione in C.
These findings suggest that dietary Cd is potentially more toxic than aqueously derived Cd in this mayfly. We further examined the effect of dietary Cd tissue loading rates on antioxidant enzyme suppression and found that absolute tissue load appeared more important than loading rate.
These results may help explain why insects are routinely unresponsive to dissolved metal exposures in the laboratory, yet highly responsive to metal pollution in nature. Parallel variation in isoenzyme and nitrogen fixation markers in a Rhizobium population. The largest group was represented 7 times according to isoenzymes and 8 times according to RFLP. This fixed non-random association of plasmid and chromosomal genotypes is consistent with a clonal population structure; it indicates limited exchange of plasmids under natural ILdriven ROS reduction is associated with an increase in the master antioxidant factor NRF2, which rapidly translocates to the mitochondria to decrease mitochondrial activity and stimulate mitophagy.
Antioxidant -enzyme reaction to the oxidative stress due to alpha-cypermethrin, chlorpyriphos, and pirimicarb in tomato Lycopersicon esculentum Mill. Tomato Lycopersicon esculentum Mill. The capacity to induce oxidative stress in tomato plant, exposed to three xenobiotics such as alpha-cypermethrin, chlorpyriphos, and pirimicarb, was investigated by the evaluation of lipid peroxidation by measuring malondialdehyde MDA rate; also, we studied the response of tomato to this stress by assessing the response of superoxide dismutase SOD , catalase CAT , peroxidase POD , ascorbate peroxidase APX , glutathione-s-transferase GST , and glutathione reductase GR.
Our results show an important accumulation of MDA, demonstrating the increase of lipid peroxidation in consequence of the excessive reactive oxygen species ROS production due to insecticide treatment. In response to this oxidative stress in tomato seedlings and plants, the activities of antioxidant -enzyme system were generally enhanced. The expression of GST isoenzymes and p53 in non-small cell lung cancer. Full Text Available This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase alpha, pi, mu, theta and p53 in non-small cell lung carcinoma and normal lung tissue from 50 patients.
The relationships between expressions of the Glutathione-S-transferase isoenzymes and some clinicopathological features were also examined.
Expression of glutathione-S-transferase pi, mu, alpha, theta and p53 was assessed by immunohistochemistry for primary lung carcinomas of 50 patients from the Sanitarium Education and Research Hospital, Ankara lung cancer collection.
The relationships between expression of the glutathione-S-transferase isoenzymes , p53 in normal and tumor tissue by Student T test and the clinicopathological data were also examined by Spearman Rank tests. When the normal and tumor tissue of these cases were compared according to their staining intensity and percentage of positive staining, glutathione-S-transferase alpha, pi, mu, theta expressions in tumor cells was significantly higher than normal cells p 0.
We have developed a microcomputer based system for interpretative reporting of creatine kinase CK and lactate dehydrogenase LDH isoenzyme studies. The test results are compared with normal range values and an interpretative report is generated. This report consists of all pertinent demographic information with a graphic display of up to 12 previous CK and LDH isoenzyme determinations. Diagnostic interpretative statements are printed beneath the graphic display following analysis of previously entered test results.
The combination of graphic data display and interpretations based on analysis of up to 12 previous specimens provides useful and accurate information to the cardiologist. Measurements of amylase isoenzymes in sera and saliva of patients after radiotherapy because of larynx carcinoma. Serum and salivary alpha-amylase were measured for controls and patients with laryngeal carcinoma before and after localized irradiation including salivary glands. A significant increase in amylasemia was observed after irradiation.
Alpha-amylase activity in saliva was decreased after irradiation but differences were not statistically significant due to the significant decrease of protein in saliva of irradiated group. An increase of salivary isoenzyme S activity was observed while pancreatic isoenzyme activity was not altered.
This method allows easy differentiation of hyperamylasemia due to irradiation of parothyroid gland and disorders of the pancreas. Alpha-amylase activity measurements may detect metabolic changes in salivary glands after irradiation.
Twenty-three isoenzyme loci had one allele only, and 16 loci had from two to five alleles. One hordein locus had Antioxidant responses to an acute ultra-endurance exercise: impact on DNA stability and indications for an increased need for nutritive antioxidants in the early recovery phase.
Antioxidant requirements have neither been defined for endurance nor been defined for ultra-endurance athletes. To verify whether an acute bout of ultra-endurance exercise modifies the need for nutritive antioxidants , we aimed 1 to investigate the changes of endogenous and exogenous antioxidants in response to an Ironman triathlon; 2 to particularise the relevance of antioxidant responses to the indices of oxidatively damaged blood lipids, blood cell compounds and lymphocyte DNA and 3 to examine whether potential time-points of increased susceptibility to oxidative damage are associated with alterations in the antioxidant status.
Blood that was collected from forty-two well-trained male athletes 2 d pre-race, immediately post-race, and 1, 5 and 19 d later was sampled. Exercise-induced changes in the plasma antioxidant capacity were associated with changes in uric acid, bilirubin and vitamin C. Furthermore, the present study illustrates the importance of a diversified and well-balanced diet to maintain a physiological antioxidant status in ultra-endurance athletes in reference to recommendations.
Genetic structuring and differentiation of Echinococcus multilocularis in Slovakia assessed by sequencing and isoenzyme studies. Nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 CO1 gene and isoenzyme analysis were used to survey the genetic variability in Echinococcus multilocularis populations from Slovakia.
A sample of 12 isolates acquired from 10 different districts from red foxes exhibited These data, along with the recently gathered data from French isolates, are indicative of a genetically unique population occurring in Central and Western Europe.
Electrophoretic examination of enzymes produced by 14 gene loci revealed intraspecific polymorphism only with the glucose Fixed genetic differences between the species were detected in the glucose-phosphate isomerase, esterase and aldolase systems, and partial differences were detected in four additional systems A systems biology perspective on Nrf2-mediated antioxidant response.
Cells in vivo are constantly exposed to reactive oxygen species ROS generated endogenously and exogenously. To defend against the deleterious consequences of ROS, cells contain multiple antioxidant enzymes expressed in various cellular compartments to scavenge these toxic species. Under oxidative stresses, these antioxidant enzymes are upregulated to restore redox homeostasis. Such an adaptive response results from the activation of a redox-sensitive gene regulatory network mediated by nuclear factor E2-related factor 2.
To more completely understand how the redox control system is designed by nature to meet homeostatic goals, we have examined the network from a systems perspective using engineering approaches. As with man-made control devices, the redox control system can be decomposed into distinct functional modules, including transducer, controller, actuator, and plant. Cells achieve specific performance objectives by utilizing nested feedback loops, feedforward control, and ultrasensitive signaling motifs, etc.
Given that endogenously generated ROS are also used as signaling molecules, our analysis suggests a novel mode of action to explain oxidative stress-induced pathological conditions and diseases. Specifically, by adaptively upregulating antioxidant enzymes, oxidative stress may inadvertently attenuate ROS signals that mediate physiological processes, resulting in aberrations of cellular functions and adverse consequences.
Lastly, by simultaneously considering the two competing cellular tasks-adaptive antioxidant defense and ROS signaling-we re-examine the premise that dietary antioxidant supplements is generally beneficial to human health. Our analysis highlights some possible adverse effects of these widely consumed antioxidants. Background A new component of the protein antioxidant capacity, designated Response Surplus RS , was recently described.
A major feature of this component is the close relationship between protein antioxidant capacity and molecular structure. Oxidative stress is associated with renal dysfunction in patients with renal failure, and plasma albumin is the target of massive oxidation in nephrotic syndrome and diabetic nephropathy. The aim of the present study was to explore the albumin redox state and the RS component of human albumin isolated from diabetic patients with progressive renal damage.
In addition to clinical and biochemical variables, the albumin redox state, including antioxidant capacity, thiol group content, and RS component, were evaluated. The albumin antioxidant capacity and thiol group content were reciprocally related to the RS component in association with GFR reduction. The findings confirm that the albumin molecular structure is closely related to its redox state, and is a key factor in the progression of diabetes nephropathy.
Extraction and antioxidation of polysaccharide from porphyra haitanensis using response surface method. This paper deals with the preparation and antioxidation of polysaccharide from Porphyra haitanensis. The ratio of water to raw material, extraction temperature and extraction time were taken in sequence as independent variables in single factor test, and polysaccharide yield as response value. Using Box-Benhnken central combination experimental design principles and response surface methodology, interactions of variables and their influence on polysaccharide yield of P.
Furthermore, the polysaccharide of P. Enhanced antioxidative responses of a salt-resistant wheat cultivar Enhanced antioxidative responses of a salt-resistant wheat cultivar facilitate its adaptation to salt stress.
Wheat cultivars capable of accumulating minerals under salt stress are of considerable interest for their potential to improve crop productivity and crop quality. This study addressed the Threonine modulates immune response , antioxidant status and gene expressions of antioxidant enzymes and antioxidant -immune-cytokine-related signaling molecules in juvenile blunt snout bream Megalobrama amblycephala.
A 9-week feeding trial was conducted to investigate the effects of graded dietary threonine Thr levels 0. For this purpose, 3 tanks were randomly arranged and assigned to each experimental diet. Fish were fed with their respective diet to apparent satiation 4 times daily. The results indicated that white blood cell, red blood cell and haemoglobin significantly responded to graded dietary Thr levels, while hematocrit didn't.
These genes are involved in different functions including antioxidant , immune, and defense responses , energy metabolism and protein synthesis. Therefore, this study could provide a new molecular tool for studies in fish immunonutrition and shed light on the regulatory mechanisms that dietary Thr improved the antioxidant and immune capacities of fish. Rab11 is phosphorylated by classical and novel protein kinase C isoenzymes upon sustained phorbol ester activation.
Rab11 is a small GTPase that controls diverse intracellular trafficking pathways. However, the molecular machinery that regulates the participation of Rab11 in those different transport events is poorly understood. In resting cells, Rab11 localizes at the endocytic recycling compartment ERC , whereas the different protein kinase C PKC isoforms display a cytosolic distribution. Although several Rabs have been shown to be phosphorylated, there is to date no evidence indicating Rab11 as a kinase substrate.
In this report, we show that Rab11 appears phosphorylated in vivo in phorbol ester-stimulated cells. In agreement, the phosphomimetic mutant, Rab11 SD, retains transferrin at the ERC in the absence of phorbolmyristateacetate stimulus. This report shows for the first time that Rab11 is differentially phosphorylated by distinct PKC isoenzymes and that this post-translational modification might be a regulatory mechanism of intracellular trafficking.
Optimization of ultrasonic extraction of phenolic antioxidants from green tea using response surface methodology. Response surface methodology RSM has been used to optimize the extraction conditions of antioxidants with relatively low caffeine content from green tea by using ultrasonic extraction. The predicted optimal conditions for the highest antioxidant activity and minimum caffeine level were found at In the predicted optimal conditions, the experimental values were very close to the predicted values.
In this study, ultrasonic extraction showed that the ethanol concentration and extraction time used for antioxidant extraction could be remarkably reduced without a decrease in antioxidant activity compared to the conventional extraction conditions. Intracellular antioxidants dissolve man-made antioxidant nanoparticles: using redox vulnerability of nanoceria to develop a responsive drug delivery system. Regeneratable antioxidant property of nanoceria has widely been explored to minimize the deleterious influences of reactive oxygen species.
Limited information is, however, available regarding the biological interactions and subsequent fate of nanoceria in body fluids. This study demonstrates a surprising dissolution of stable and ultrasmall 4 nm cerium oxide nanoparticles CeO2 NPs in response to biologically prevalent antioxidant molecules glutathione, vitamin C.
Such a redox sensitive behavior of CeO2 NPs is subsequently exploited to design a redox responsive drug delivery system for transporting anticancer drug camptothecin. Upon exposing the CeO2 capped and drug loaded nanoconstruct to vitamin c or glutathione, dissolution-accompanied aggregation of CeO2 nanolids unleashes the drug molecules from porous silica to achieve a significant anticancer activity. Besides stimuli responsive drug delivery, immobilization of nanoceria onto the surface of mesoporous silica also facilitates us to gain a basic insight into the biotransformation of CeO2 in physiological mediums.
Immunohistochemical detection of cytochrome P isoenzymes in cultured human epidermal cells. We used specific monoclonal antibodies MAb to human cytochrome P isoenzymes to determine the presence of these proteins in human epidermal cells. Two MAb P and P recognize major forms of hepatic cytochrome P involved in biotransformation of xenobiotics.
A third MAb, to cytochrome P, is not fully characterized. The proteins were determined by the indirect immunoperoxidase technique after fixation with methanol and acetone. Biopsy materials for cultured keratinocytes, i. In cultured keratinocytes derived from hair follicles the proteins were undetectable, whereas the keratinocytes derived from foreskin continued to express the two major forms of hepatic cytochrome P Cultured human fibroblasts and a human keratinocyte cell line SVK14 showed staining similar to that of the foreskin keratinocytes.
Cytochrome P was detectable only in human hepatocytes. The results indicate that, under the culture conditions applied, cultured human foreskin cells and the cell line SVK14 continue to express specific cytochrome P isoenzymes in culture, in contrast to hair follicle keratinocytes. Studies on the effects on growth and antioxidant responses of two marine microalgal species to uniconazole. Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase SOD and peroxidase POD enzymes that retard lipid peroxidation and membrane deterioration.
These years much attention has been focused on the responses of antioxidant system in plants to uniconazole stress, but such studies on aquatic organism are very few. Moreover, no information is available on growth and antioxidant response in marine microalgae to uniconazole.
In this paper, the growth and antioxidant responses of two marine microalgal species, Platymonas helgolandica and Pavlova viridis, at six uniconazole concentrations mg L-1 were investigated. The results demonstrated that 3 mg L-1 uniconazole could increase significantly chlorophyll a and carbohydrate contents of P. Sublethal toxicity of quinalphos on oxidative stress and antioxidant responses in a freshwater fish Cyprinus carpio.
Extensive use of quinalphos, an organophosphorus pesticide, is likely to reach the aquatic environment and thereby posing a health concern for aquatic organisms. Oxidative stress and antioxidant responses may be good indicators of pesticide contamination in aquatic organisms.
The data on quinalphos induced oxidative stress and antioxidant responses in carps are scanty. This study is aimed to assess the two sublethal concentrations of quinalphos 1. In liver, the malondialdehyde level was found to be significantly increased in both the concentrations. The results of the antioxidant parameters obtained show a significant increase in superoxide dismutase, catalase, and glutathione-S-transferase activity in liver of fish.
These results demonstrate that environmentally relevant levels of the insecticide quinalphos can cause oxidative damage and increase the antioxidant scavenging capacity in C. This may reflect the potential role of these parameters as useful biomarkers for the assessment of pesticide contamination. Environ Toxicol , Full Text Available Response surface methodology RSM has been used to optimize the extraction conditions of antioxidants with relatively low caffeine content from green tea by using ultrasonic extraction.
Measurement of the total antioxidant response in preeclampsia with a novel automated method. Preeclampsia is one of the most serious complications of pregnancy. Free radical damage has been implicated in the pathophysiology of this condition. In this study, we aimed to measure the antioxidant capacity in plasma samples from normotensive and preeclamptic pregnant women to evaluate their antioxidant status using a more recently developed automated measurement method.
Our study group contained 42 women, 24 of whom had preeclampsia, while 18 had normotensive pregnancies. We measured the total plasma antioxidant capacity for all patients, as well as the levels of four major individual plasma antioxidant components; albumin, uric acid, ascorbic acid and bilirubin, and as a reciprocal measure, their total plasma peroxide levels. Statistically significant differences determined using Student's t-test were noted between the normotensive and the preeclamptic groups for their total antioxidant responses and their vitamin C levels 1.
In contrast, the total plasma peroxide levels were significantly elevated in this group We found a decreased total antioxidant response in preeclamptic patients using a simple, rapid and reliable automated colorimetric assay, which may suitable for use in any routine clinical biochemistry laboratory, and considerably facilitates the assessment of this useful clinical parameter.
We suggest that this novel method may be used as a routine test to evaluate and follow up of the levels of oxidative stress in preeclampsia. Preliminary study on peroxidase isoenzyme detection and RAPD molecular verification for sweet pepper carried by a recoverable satellite. The detection technology of peroxidase isoenzyme pattern and RAPD Randomly Amplified Polymorphic DNA analysis were used to space-flown sweet pepper variety of carried by a recoverable satellite, and its correspondent ground control Longjiao Determination of peroxidase isoenzyme revealed that the physiological activities of space-flown sweet pepper were significantly higher than those of its ground control.
To detect the polymorphism of gene sequence and the difference in molecular level. Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi. Despite high sequence similarity at the amino acid residue level, the recombinant isoenzymes show a strikingly different kinetics. Whereas TcRPE2 follows a typical michaelian behavior, TcRPE1 shows a complex kinetic pattern, displaying a biphasic curve, suggesting the coexistence of -at least- two kinetically different molecular forms.
With regard to the architecture of the active site, all the important amino acid residues for catalysis -with the exception of M are also present in both TcRPEs models.
The superimposition of the binding pocket of both isoenzyme models shows that they adopt essentially identical positions in the active site with a residue specific RMSD isoenzymes reveal that both are present in a mixture of various oligomeric species made up of an even number of molecules, probably pointing to the dimer as their minimal functional unit.
This multiplicity of oligomeric species has not been reported for any of the other RPEs studied so far and it might bear implications for the regulation of TcRPEs activity, although further investigation will be necessary to unravel the physiological.
UV-induced changes of structural and functional properties of blood lactatedehydrogenase isoenzymes in free sate and in the presence of serotonin.
Photoinduced changes in structural and functional properties of lactatedehydrogenase isoenzymes from human erythrocytes in free state and in the presence of serotonin have been studied by means of gel chromatography, electrophoresis, IR-spectrophotometry and by the method of definition of catalytic activity.
UV radiation exposure induces photoinactivation of erythrocytes lactatedehydrogenase, while its inhibitory effect intensifies with the increase of radiation dose. Pronounced photoprotective effect of serotonin towards the molecules of erythrocytic lactatedehydrogenase isoenzymes has been discovered. It seems to be caused by formation of enzyme - biogenic amine complex affecting the secondary protein structure [ru.
Herbicidal and antioxidant responses of transgenic rice overexpressing Myxococcus xanthus protoporphyrinogen oxidase.
We analyzed the herbicidal and antioxidant defense responses of transgenic rice plants that overexpressed the Myxococcus xanthus protoporphyrinogen oxidase gene. Leaf squares of the wild-type incubated with oxyfluorfen were characterized by necrotic leaf lesions and increases in conductivity and malonyldialdehyde levels, whereas transgenic lines M4 and M7 did not show any change with up to microM oxyfluorfen.
In response to oxyfluorfen, violaxanthin, beta-carotene, and chlorophylls Chls decreased in wild-type plants, whereas antheraxanthin and zeaxanthin increased. Only a slight decline in Chls was observed in transgenic lines at 48 h after oxyfluorfen treatment. Non-enzymatic antioxidants appeared to respond faster to oxyfluorfen-induced photodynamic stress than did enzymatic antioxidants.
Protective responses for the detoxification of active oxygen species were induced to counteract photodynamic stress in oxyfluorfen-treated, wild-type plants. However, oxyfluorfen-treated, transgenic plants suffered less oxidative stress, confirming increased herbicidal resistance resulted from dual expression of M. Rosmarinic acid and antioxidant enzyme activities in Lavandula vera MM cell suspension culture: a comparative study. The growth and intracellular protein content of lavender Lavandula vera MM cell suspension culture was followed along with some antioxidant defense system members-non-enzymatic rosmarinic acid and enzymatic [superoxide dismutase EC 1.
It was found that the media content and the cultivation mode strongly influenced the production of plant defense compounds as well as the ratio between non-enzymatic and enzymatic ones. The bioreactor culture contains about two times more rosmarinic acid, superoxide dismutase, and catalase compared to the shake-flask cultivation. These findings are discussed with respect to the relative stress levels and plant antioxidant orchestra system.
It was concluded that investigated defense system components enzymatic and non-enzymatic were closely associated in a complex balance. Study for identification of beneficial uses of Space BUS. Volume 2: Technical report. Book 1: Development and business analysis of space processed isoenzymes. A separation method to provide reasonable yields of high specificity isoenzymes for the purpose of large scale, early clinical diagnosis of diseases and organic damage such as, myocardial infarction, hepatoma, muscular dystrophy, and infectous disorders is presented.
Preliminary development plans are summarized. An analysis of required research and development and production resources is included. The costs of such resources and the potential profitability of a commercial space processing opportunity for electrophoretic separation of high specificity isoenzymes are reviewed.
The role of 11? Full Text Available The action of glucocorticoids is modulated by isoenzymes 11? With the aim at describing the action of isoenzymes 11? Review studies, meta-analysis and original articles were selected and chosen on the basis of methodological aspects and relevance. One person had asked an interesting question to Blind Mew.
There are gorillas of a similar type in both Monquista namely Gortez and Zafaria. Which world did they originate in? Blind Mew answered:. Good question. I hadn't really thought that through. Gortez is prominent enough in Monquistan culture that I doubt they would have conferred such honors on anybody not native born - if I had to wager, I'd bet he's from Monquista. As to the origins of Zafarian vs. Monquistan Gorillas, I'm content to leave that vague.
Migration is definitely one possibility, though there could just have been apes in two places when the First World shattered. Gortez also seems to be the only gorilla of his kind in Monquista.
Perhaps that tells us something. One thing I can't quite figure out is why we don't see Zafaria in the Marleybone stormgate. That would make it appear such that sailing all the way from Marleybone to Zafaria would be quite the task.
One way or another, I'm beginning to pay more attention to these details with Pirate, and seeing how the two stories work together to give details about the other. If you're a wizard or pirate, and not both, scroll up and click on the play buttons on the site! Give the other game a try. You might like it. Thanks for reading and see you in
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